CFR › Title 49 › Part 40 › §40.8249 CFR §40.82Verified against eCFR.gov as of June 20, 2026View official text on eCFR.gov ↗Copy linkAs a laboratory, you must test for the following five drugs or classes of drugs in a DOT drug test. You must not test “DOT specimens” for any other drugs.(a)Marijuana metabolites.(b)Cocaine metabolites.(c)Amphetamines.(d)Opioids.(e)Phencyclidine (PCP).