27 CFR § 21.38
Formula No. 4
April 24, 2020
(a) Formula. To every 100 gallons of alcohol, or to every 100 gallons of rum of not less than 150 proof, add:
One gallon of the following solution: Five gallons of an aqueous solution containing 40 percent nicotine; 3.6 avoirdupois ounces of methylene blue, U.S.P.; and water sufficient to make 100 gallons.
(b) Authorized uses.
(1) As a solvent:
460. Tobacco sprays and flavors.
(2) Miscellaneous uses:
812. Product development and pilot plant uses (own use only).