40 CFR Appendix A to Subpart C of Part 141 Alternative Testing Methods Approved for Analyses Under the Safe Drinking Water Act | Code of Federal Regulations | US Law

40 CFR Appendix A to Subpart C of Part 141

Alternative Testing Methods Approved for Analyses Under the Safe Drinking Water Act

June 9, 2020

CFR

Only the editions stated in the following table are approved.

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.21(f)(3)

Organism	Methodology	SM 21st Edition¹	SM 22nd Edition²⁸	SM Online³	Other
Total Coliforms	Total Coliform Fermentation Technique	9221 A, B	9221 A, B	9221 A,B-06
	Total Coliform Membrane Filter Technique	9222 A, B, C
	Presence-Absence (P-A) Coliform Test	9221 D
	ONPG-MUG Test	9223	9223 B	9223 B-04
	Colitag^TM				Modified Colitag^TM¹³
	Tecta EC/TC³³

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.21(f)(5)

Open Table

Organism	Methodology	SM 22nd Edition²⁸	SM Online³
Fecal Coliforms	Fecal Coliform Procedure	9221 E	9221 E-06

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.21(f)(6)

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Organism	Methodology	SM 20th Edition⁶	SM 21st Edition¹	SM 22nd Edition²⁸	SM Online³	Other
E.coli	ONPG-MUG Test	9223 B	9223 B	9223 B	9223 B-97, B-04
	Colitag^TM					Modified Colitag^TM¹³
	Tecta EC/TC³³

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.23(k)(1)

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Contaminant	Methodology	EPA method	SM 21st edition¹	SM 22nd edition²⁸	SM 23rd edition⁴⁹	SM Online³	ASTM⁴	Other
Alkalinity	Titrimetric		2320 B	2320 B	2320 B		D1067-06 B, 11 B, 16 B
Antimony	Hydride—Atomic Absorption						D 3697-07, -12
	Atomic Absorption; Furnace		3113 B	3113 B	3113 B	3113 B-04, B-10
	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2²
Arsenic	Atomic Absorption; Furnace		3113 B	3113 B	3113 B	3113 B-04, B-10	D 2972-08 C, -15 C
	Hydride Atomic Absorption		3114 B	3114 B	3114 B	3114 B-09	D 2972-08 B, -15 B
	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2²
Barium	Inductively Coupled Plasma		3120 B	3120 B	3120 B
	Atomic Absorption; Direct		3111 D	3111 D	3111 D
	Atomic Absorption; Furnace		3113 B	3113 B	3113 B	3113 B-04, B-10
	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2²
Beryllium	Inductively Coupled Plasma		3120 B	3120 B	3120 B
	Atomic Absorption; Furnace		3113 B	3113 B	3113 B	3113 B-04, B-10	D 3645-08 B, -15 B
	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2²
Cadmium	Atomic Absorption; Furnace		3113 B	3113 B	3113 B	3113 B-04, B-10
	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2²
Calcium	EDTA titrimetric		3500-Ca B	3500-Ca B	3500-Ca B		D 511-09, -14 A
	Atomic Absorption; Direct Aspiration		3111 B	3111 B	3111 B		D 511-09, -14 B
	Inductively Coupled Plasma		3120 B	3120 B	3120 B
	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2²
	Ion Chromatography						D 6919-09
Chromium	Inductively Coupled Plasma		3120 B	3120 B	3120 B
	Atomic Absorption; Furnace		3113 B	3113 B	3113 B	3113 B-04, B-10
	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2²
Copper	Atomic Absorption; Furnace		3113 B	3113 B	3113 B	3113 B-04, B-10	D 1688-07, -12 C
	Atomic Absorption; Direct Aspiration		3111 B	3111 B	3111 B		D 1688-07, -12 A
	Inductively Coupled Plasma		3120 B	3120 B	3120 B
	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2²
	Colorimetry							Hach Method 8026;³⁵ Hach Method 10272.³⁶
Conductivity	Conductance		2510 B	2510 B	2510 B		D 1125-14 A
Cyanide	Manual Distillation with MgCl2 followed by:		4500-CN− C	4500-CN− C	4500-CN− C	4500-CN− C-99	D 2036-06 A
	Spectrophotometric, Amenable		4500-CN− G	4500-CN− G	4500-CN− G		D 2036-06 B
	Spectrophotometric Manual		4500-CN− E	4500-CN− E	4500-CN− E		D2036-06 A
	Selective Electrode		4500-CN− F	4500-CN− F	4500-CN− F
	Gas Chromatography/Mass Spectrometry Headspace							ME355.01.⁷
Fluoride	Ion Chromatography		4110 B	4110 B	4110 B		D 4327-11
	Manual Distillation; Colorimetric SPADNS		4500-F− B, D	4500-F− B, D	4500-F− B, D
	Manual Electrode		4500-F− C	4500-F− C	4500-F− C		D 1179-04, 10 B, 16 B
	Automated Alizarin		4500-F− E	4500-F− E	4500-F− E
	Arsenite-Free Colorimetric SPADNS							Hach SPADNS 2 Method 10225.²²
Lead	Atomic Absorption; Furnace		3113 B	3113 B	3113 B	3113 B-04, B-10	D 3559-08 D, 15 D
	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2²
Magnesium	Atomic Absorption		3111 B	3111 B	3111 B		D 511-09, -14 B
	Inductively Coupled Plasma		3120 B	3120 B	3120 B
	Complexation Titrimetric Methods		3500-Mg B	3500-Mg B	3500-Mg B		D 511-09, -14 A
	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2²
	Ion Chromatography						D 6919-09
Mercury	Manual, Cold Vapor		3112 B	3112 B	3112 B	3112 B-09	D 3223-12
Nickel	Inductively Coupled Plasma		3120 B	3120 B	3120 B
	Atomic Absorption; Direct		3111 B	3111 B	3111 B
	Atomic Absorption; Furnace		3113 B	3113 B	3113 B	3113 B-04, B-10
	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2²
Nitrate	Ion Chromatography		4110 B	4110 B	4110 B		D 4327-11
	Automated Cadmium Reduction		4500-NO3− F	4500-NO3− F	4500-NO3− F
	Manual Cadmium Reduction		4500-NO3− E	4500-NO3− E	4500-NO3− E
	Ion Selective Electrode		4500-NO3− D	4500-NO3− D	4500-NO3− D
	Reduction/Colorimetric							Systea Easy (1-Reagent);⁸ NECi Nitrate-Reductase.⁴⁰
	Colorimetric; Direct							Hach TNTplus^TM 835/836 Method 10206.²³
	Capillary Ion Electrophoresis						D 6508-15
Nitrite	Ion Chromatography		4110 B	4110 B	4110 B		D 4327-11
	Automated Cadmium Reduction		4500-NO3− F	4500-NO3− F	4500-NO3− F
	Manual Cadmium Reduction		4500-NO3− E	4500-NO3− E	4500-NO3− E
	Spectrophotometric		4500-NO2− B	4500-NO2− B	4500-NO2− B
	Reduction/Colorimetric							Systea Easy (1-Reagent);⁸ NECi Nitrate-Reductase.⁴⁰
	Capillary Ion Electrophoresis						D 6508-15
Ortho-phosphate	Ion Chromatography		4110 B	4110 B	4110 B		D 4327-11
	Colorimetric, ascorbic acid, single reagent		4500-P E	4500-P E	4500-P E	4500-P E-99
	Colorimetric, Automated, Ascorbic Acid		4500-P F	4500-P F	4500-P F	4500-P F-99		Thermo Fisher Discrete Analyzer.⁴¹
	Capillary Ion Electrophoresis						D 6508-15
pH	Electrometric	150.3⁴⁸	4500-H+ B	4500-H+ B	4500-H+ B		D 1293-12
Selenium	Hydride-Atomic Absorption		3114 B	3114 B	3114 B	3114 B-09	D 3859-08 A, -15 A
	Atomic Absorption; Furnace		3113 B	3113 B	3113 B	3113 B-04, B-10	D 3859-08 B, -15 B
	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2²
Silica	Colorimetric						D859-05, 10, 16
	Molybdosilicate		4500-SiO2 C	4500-SiO2 C	4500-SiO2 C
	Heteropoly blue		4500-SiO2 D	4500-SiO2 D	4500-SiO2 D
	Automated for Molybdate-reactive Silica		4500-SiO2 E	4500-SiO2 E	4500-SiO2 E
	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2²
	Inductively Coupled Plasma		3120 B	3120 B	3120 B
Sodium	Atomic Absorption; Direct Aspiration		3111 B	3111 B	3111 B
	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2²
	Ion Chromatography						D 6919-09
Temperature	Thermometric		2550	2550	2550	2550-10

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.24(e)(1)

Open Table

Contaminant	Methodology	EPA method	SM 21st edition¹	SM 22nd edition,²⁸ SM 23rd edition⁴⁹	SM online³
Benzene	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
Carbon tetrachloride	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
Chlorobenzene	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
1,2-Dichlorobenzene	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
1,4-Dichlorobenzene	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
1,2-Dichloroethane	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
cis-Dichloroethylene	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
trans-Dichloroethylene	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
Dichloromethane	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
1,2-Dichloropropane	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
Ethylbenzene	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
Styrene	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,;⁹ 524.4.²⁹
Tetrachloroethylene	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
1,1,1-Trichloroethane	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
Trichloroethylene	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
Toluene	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
1,2,4-Trichlorobenzene	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
1,1-Dichloroethylene	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
1,1,2-Trichlorethane	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
Vinyl chloride	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
Xylenes (total)	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹ 524.4.²⁹
2,4-D	Gas Chromatography/Electron Capture Detection (GC/ECD)		6640 B	6640 B	6640 B-01, B-06.
2,4,5-TP (Silvex)	Gas Chromatography/Electron Capture Detection (GC/ECD)		6640 B	6640 B	6640 B-01, B-06.
Alachlor	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Atrazine	Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC/ESI-MS/MS)	536.²⁵
	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3,²⁴523.²⁶
Benzo(a)pyrene	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Carbofuran	High-performance liquid chromatography (HPLC) with post-column derivatization and fluorescence detection		6610 B	6610 B	6610 B-04.
Chlordane	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Dalapon	Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS)	557.¹⁴
	Gas Chromatography/Electron Capture Detection (GC/ECD)		6640 B	6640 B	6640 B-01, B-06.
Di(2-ethylhexyl)adipate	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Di(2-ethylhexyl)phthalate	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Dibromochloropropane (DBCP)	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3.⁹
Dinoseb	Gas Chromatography/Electron Capture Detection (GC/ECD)		6640 B	6640 B	6640 B-01, B-06.
Endrin	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Ethyl dibromide (EDB)	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3.⁹
Glyphosate	High-Performance Liquid Chromatography (HPLC) with Post-Column Derivatization and Fluorescence Detection		6651 B	6651 B	6651 B-00, B-05.
Heptachlor	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Heptachlor Epoxide	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Hexachlorobenzene	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Hexachlorocyclo-pentadiene	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Lindane	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Methoxychlor	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Oxamyl	High-performance liquid chromatography (HPLC) with post-column derivatization and fluorescence detection		6610 B	6610 B	6610 B-04.
PCBs (as Aroclors)	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Pentachlorophenol	Gas Chromatography/Electron Capture Detection (GC/ECD)		6640 B	6640 B	6640 B-01, B-06.
	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Picloram	Gas Chromatography/Electron Capture Detection (GC/ECD)		6640 B	6640 B	6640 B-01, B-06.
Simazine	Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC/ESI-MS/MS)	536.²⁵
	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3,²⁴523.²⁶
Toxaphene	Solid Phase Extraction/Gas Chromatography/Mass Spectrometry (GC/MS)	525.3.²⁴
Total Trihalomethanes	Purge &Trap/Gas Chromatography/Mass Spectrometry	524.3,⁹524.4²⁹

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.25(a)

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Contaminant	Methodology	EPA method	SM 21st edition¹	SM 22nd edition,²⁸ SM 23rd edition⁴⁹	ASTM⁴	SM Online³
Naturally Occurring:
Gross alpha and beta	Evaporation	900.0, Rev. 1.0⁵⁰	7110 B	7110 B
	Liquid Scintillation				D 7283-17	7110 D-17.
Gross alpha	Coprecipitation		7110 C	7110 C
Radium 226	Radon emanation		7500-Ra C	7500-Ra C	D 3454-05
	Radiochemical		7500-Ra B	7500-Ra B	D 2460-07
	Gamma Spectrometry			7500-Ra E		7500-Ra E-07.
Radium 228	Radiochemical		7500-Ra D	7500-Ra D
	Gamma Spectrometry			7500Ra-E		7500-Ra E-07.
Uranium	Radiochemical		7500-U B	7500-U B
	ICP-MS		3125		D 5673-05, 10, 16
	Alpha spectrometry		7500-U C	7500-U C	D 3972-09
	Laser Phosphorimetry				D 5174-07
	Alpha Liquid Scintillation Spectrometry				D 6239-09
Man-Made:
Radioactive Cesium	Radiochemical		7500-Cs B	7500-Cs B
	Gamma Ray Spectrometry		7120	7120	D 3649-06
Radioactive Iodine	Radiochemical		7500-I B	7500-I B	D 3649-06
			7500-I C	7500-I C
			7500-I D	7500-I D
	Gamma Ray Spectrometry		7120	7120	D 4785-08
Radioactive Strontium 89, 90	Radiochemical		7500-Sr B	7500-Sr B
Tritium	Liquid Scintillation		7500-³H B	7500-³H B	D 4107-08
Gamma Emitters	Gamma Ray		7120	7120	D 3649-06
	Spectrometry		7500-Cs B	7500-Cs B	D 4785-08
			7500-I B	7500-I B

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.74(a)(1)

Open Table

Organism	Methodology	SM 21st edition¹	SM 22nd edition²⁸	SM 23rd edition⁴⁹	SM Online³	Other
Total Coliform	Total Coliform Fermentation Technique	9221 A, B, C	9221 A, B, C	9221 A, B, C	9221 A,B,C-06
	Total Coliform Membrane Filter Technique	9222 A, B, C		9222 A, B, C.
	ONPG-MUG Test	9223	9223 B	9223 B	9223 B-04.
Fecal Coliforms	Fecal Coliform Procedure	9221 E	9221 E	9221 E	9221 E-06.
	Fecal Coliform Filter Procedure	9222 D	9222 D	9222 D	9222 D-06.
Heterotrophic bacteria	Pour Plate Method	9215 B	9215 B	9215 B	9215 B-04.
Turbidity	Nephelometric Method	2130 B	2130 B	2130 B		Hach Method 8195, Rev. 3.0.⁵²
	Laser Nephelometry (on-line)					Mitchell M5271,¹⁰ Mitchell M5331, Rev. 1.2,⁴² Lovibond PTV 6000.⁴⁶
	LED Nephelometry (on-line)					Mitchell M5331,¹¹ Mitchell M5331, Rev. 1.2,⁴² Lovibond PTV 2000.⁴⁵
	LED Nephelometry (on-line)					AMI Turbiwell,¹⁵ Lovibond PTV 1000.⁴⁴
	LED Nephelometry (portable)					Orion AQ4500.¹²
	360° Nephelometry					Hach Method 10258 Rev. 1.0,³⁹ Hach Method 10258, Rev. 2.0.⁵¹

Alternative Testing Methods for Disinfectant Residuals Listed at 40 CFR 141.74(a)(2)

Open Table

Residual	Methodology	SM 21st edition¹	SM 22nd edition,²⁸ SM 23rd edition⁴⁹	ASTM⁴	Other
Free Chlorine	Amperometric Titration	4500-Cl D	4500-Cl D	D 1253-08, -14
	DPD Ferrous Titrimetric	4500-Cl F	4500-Cl F
	DPD Colorimetric	4500-Cl G	4500-Cl G		Hach Method 10260.³¹
	Indophenol Colorimetric				Hach Method 10241.³⁴
	Syringaldazine (FACTS)	4500-Cl H	4500-Cl H
	On-line Chlorine Analyzer				EPA 334.0.¹⁶
	Amperometric Sensor				ChloroSense.¹⁷
Total Chlorine	Amperometric Titration	4500-Cl D	4500-Cl D	D 1253-08, -14
	Amperometric Titration (Low level measurement)	4500-Cl E	4500-Cl E.
	DPD Ferrous Titrimetric	4500-Cl F	4500-Cl F.
	DPD Colorimetric	4500-Cl G	4500-Cl G		Hach Method 10260.³¹
	Iodometric Electrode	4500-Cl I	4500-Cl I.
	On-line Chlorine Analyzer				EPA 334.0.¹⁶
	Amperometric Sensor				ChloroSense.¹⁷
Chlorine Dioxide	Amperometric Titration	4500-ClO2 C	4500-ClO2 C.
	Amperometric Titration	4500-ClO2 E.	4500-ClO2 E.
	Amperometric Sensor				ChlordioX Plus.³²
Ozone	Indigo Method	4500-O3 B	4500-O3 B.

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.131(b)(1)

Open Table

Contaminant	Methodology	EPA method	ASTM⁴	SM online³	SM 21st edition¹	SM 22nd edition,²⁸ SM 23rd edition⁴⁹	Other
TTHM	P&T/GC/MS	524.3,⁹ 524.4.²⁹
HAA5	LLE (diazomethane)/GC/ECD			6251 B-07	6251 B	6251 B.
	Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS)	557.¹⁴
	Two-Dimensional Ion Chromatography (IC) with Suppressed Conductivity Detection						Thermo Fisher 557.1.⁴⁷
Bromate	Two-Dimensional Ion Chromatography (IC)	302.0.¹⁸
	Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS)	557.¹⁴
	Chemically Suppressed Ion Chromatography		D 6581-08 A.
	Electrolytically Suppressed Ion Chromatography		D 6581-08 B.
Chlorite	Chemically Suppressed Ion Chromatography		D 6581-08 A.
	Electrolytically Suppressed Ion Chromatography		D 6581-08 B.
Chlorite—daily monitoring as prescribed in 40 CFR 141.132(b)(2)(i)(A)	Amperometric Titration				4500-ClO2 E	4500-ClO2 E.
	Amperometric Sensor						ChlordioX Plus.³²

Alternative Testing Methods for Disinfectant Residuals Listed at 40 CFR 141.131(c)(1)

Open Table

Residual	Methodology	SM 21st edition¹	SM 22nd edition,²⁸ SM 23rd edition⁴⁹	ASTM⁴	Other
Free Chlorine	Amperometric Titration	4500-Cl D	4500-Cl D	D 1253-08, -14.
	DPD Ferrous Titrimetric	4500-Cl F	4500-Cl F
	DPD Colorimetric	4500-Cl G	4500-Cl G		Hach Method 10260.³¹
	Indophenol Colorimetric				Hach Method 10241.³⁴
	Syringaldazine (FACTS)	4500-Cl H	4500-Cl H.
	Amperometric Sensor				ChloroSense.¹⁷
	On-line Chlorine Analyzer				EPA 334.0.¹⁶
Combined Chlorine	Amperometric Titration	4500-Cl D	4500-Cl D	D 1253-08, -14.
	DPD Ferrous Titrimetric	4500-Cl F	4500-Cl F
	DPD Colorimetric	4500-Cl G	4500-Cl G		Hach Method 10260.³¹
Total Chlorine	Amperometric Titration	4500-Cl D	4500-Cl D	D 1253-08, -14.
	Low level Amperometric Titration	4500-Cl E	4500-Cl E.
	DPD Ferrous Titrimetric	4500-Cl F	4500-Cl F.
	DPD Colorimetric	4500-Cl G	4500-Cl G		Hach Method 10260.³¹
	Iodometric Electrode	4500-Cl I	4500-Cl I.
	Amperometric Sensor				ChloroSense.¹⁷
	On-line Chlorine Analyzer				EPA 334.0.¹⁶
Chlorine Dioxide	Amperometric Method II	4500-ClO2 E	4500-ClO2 E.
	Amperometric Sensor				ChlordioX Plus.³²

Alternative Testing Methods for Disinfectant Residuals Listed at 40 CFR 141.131(c)(2), If Approved by the State

Open Table

Residual	Methodology	Method
Free Chlorine	Test Strips	Method D99-003⁵

Alternative Testing Methods for Parameters Listed at 40 CFR 141.131(d)

Open Table

Parameter	Methodology	SM 21st edition¹	SM 22nd edition²⁸	SM 23rd edition⁴⁹	SM online³	EPA	Other
Total Organic Carbon (TOC)	High Temperature Combustion	5310 B	5310 B	5310 B		415.3, Rev 1.2.¹⁹
	Persulfate-Ultraviolet or Heated Persulfate Oxidation	5310 C	5310 C	5310 C		415.3, Rev 1.2.¹⁹	Hach Method 10267.³⁸
	Wet Oxidation	5310 D	5310 D			415.3, Rev 1.2.¹⁹
	Ozone Oxidation						Hach Method 10261.³⁷
Specific Ultraviolet Absorbance (SUVA)	Calculation using DOC and UV254 data					415.3, Rev 1.2.¹⁹
Dissolved Organic Carbon (DOC)	High Temperature Combustion	5310 B	5310 B	5310 B		415.3, Rev 1.2.¹⁹
	Persulfate-Ultraviolet or Heated Persulfate Oxidation	5310 C	5310 C	5310 C		415.3, Rev 1.2.¹⁹
	Wet Oxidation	5310 D	5310 D			415.3, Rev 1.2.¹⁹
Ultraviolet absorption at 254 nm (UV254)	Spectrophotometry	5910 B	5910 B	5910 B	5910 B-11	415.3, Rev 1.2.¹⁹

Alternative Testing Methods With MRL ≤0.0010 mg/L for Monitoring Listed at 40 CFR 141.132(b)(3)(ii)(B)

Open Table

Contaminant	Methodology	EPA method
Bromate	Two-Dimensional Ion Chromatography (IC)	302.0¹⁸
	Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS)	557¹⁴

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.402(c)(2)

Open Table

Organism	Methodology	SM 20th edition⁶	SM 21st edition¹	SM 22nd edition²⁸	SM 23rd edition⁴⁹	SM online³	Other
E. coli	Colilert		9223 B	9223 B	9223 B	9223 B-97, B-04.
	Colisure		9223 B	9223 B	9223 B	9223 B-97, B-04.
	Colilert-18	9223 B	9223 B	9223 B	9223 B	9223 B-97, B-04.
	Readycult®						Readycult®.²⁰
	Colitag						Modified Colitag.TM¹³
	Chromocult®						Chromocult®.²¹
	EC-MUG			9221 F	9221 F	9221 F-06.
	NA-MUG				9222 I.
	m-ColiBlue24 Test				9222 J.
	Tecta EC/TC ³³⁴³
Enterococci	Multiple-Tube Technique					9230 B-04.
	Membrane Filter Techniques				9230 C.
	Fluorogenic Substrate Enterococcus Test (using Enterolert)				9230 D.
Coliphage	Two-Step Enrichment Presence-Absence Procedure						Fast Phage.³⁰

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.704(a)

Open Table

Organism	Methodology	EPA Method
Cryptosporidium	Filtration/Immunomagnetic Separation/Immunofluorescence Assay Microscopy	1623.1²⁷

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.704(b)

Open Table

Organism	Methodology	SM 20th edition⁶
E. coli	Membrane Filtration, Two Step	9222 D/9222 G

Alternative Testing Methods for Contaminants Listed at 40 CFR 141.852(a)(5)

Open Table

Organism	Methodology category	Method	SM 20th, 21st editions ^{1 6}	SM 22nd edition²⁸	SM 23rd edition⁴⁹	SM online³
Total Coliforms	Lactose Fermentation Methods	Standard Total Coliform Fermentation Technique		9221 B.1, B.2	9221 B.1, B.2, B.3, B.4	9221 B.1, B.2-06.
		Presence-Absence (P-A) Coliform Test			9221 D.1, D.2, D.3
	Membrane Filtration Methods	Standard Total Coliform Membrane Filter Procedure using Endo Media			9222 B, C.
		Simultaneous Detection of Total Coliforms and E. coli by Dual Chromogen Membrane Filter Procedure (using mColiBlue24 medium)			9222 J.
	Enzyme Substrate Methods	Colilert®		9223 B	9223 B	9223 B-04.
		Colisure®		9223 B	9223 B	9223 B-04.
		Colilert-18	9223 B	9223 B	9223 B	9223 B-04.
		Tecta EC/TC ^{33 43}
Escherichia coli	Escherichia coli Procedure (following Lactose Fermentation Methods)	EC-MUG medium		9221 F.1	9221 F.1	9221 F.1-06.
	Escherichia coli Partitioning Methods (following Membrane Filtration Methods)	EC broth with MUG (EC-MUG)			9222 H.
		NA-MUG medium			9222 I.
	Simultaneous Detection of Total Coliforms and E. coli by Dual Chromogen Membrane Filter Procedure	mColiBlue24 medium			9222 J.
	Enzyme Substrate Methods	Colilert®		9223 B	9223 B	9223 B-04.
		Colisure®		9223 B	9223 B	9223 B-04.
		Colilert-18	9223 B	9223 B	9223 B	9223 B-04.
		Tecta EC/TC ^{33 43}

Alternative Testing Methods for Contaminants Listed at 40 CFR 143.4(b)

Open Table

Contaminant	Methodology	EPA method	ASTM⁴	SM 21st edition¹	SM 22nd edition,²⁸ SM 23rd edition⁴⁹	SM online³
Aluminum	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2.²
	Atomic Absorption; Direct			3111 D	3111 D.
	Atomic Absorption; Furnace			3113 B	3113 B	3113 B-04, B-10.
	Inductively Coupled Plasma			3120 B	3120 B.
Chloride	Silver Nitrate Titration		D 512-04 B, 12 B	4500-Cl− B	4500-Cl− B
	Ion Chromatography		D 4327-11	4110 B	4110 B.
	Potentiometric Titration			4500-Cl− D	4500-Cl− D.
Color	Visual Comparison			2120 B	2120 B.
Foaming Agents	Methylene Blue Active Substances (MBAS)			5540 C	5540 C.
Iron	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2.²
	Atomic Absorption; Direct			3111 B	3111 B.
	Atomic Absorption; Furnace			3113 B	3113 B	3113 B-04, B-10.
	Inductively Coupled Plasma			3120 B	3120 B.
Manganese	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2.²
	Atomic Absorption; Direct			3111 B	3111 B.
	Atomic Absorption; Furnace			3113 B	3113 B	3113 B-04, B-10.
	Inductively Coupled Plasma			3120 B	3120 B.
Odor	Threshold Odor Test			2150 B	2150 B.
Silver	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2.²
	Atomic Absorption; Direct			3111 B	3111 B.
	Atomic Absorption; Furnace			3113 B	3113 B	3113 B-04, B-10.
	Inductively Coupled Plasma			3120 B	3120 B.
Sulfate	Ion Chromatography		D 4327-11	4110 B	4110 B.
	Gravimetric with ignition of residue			4500-SO4 2− C	4500-SO4 2− C	4500-SO4 2− C-97.
	Gravimetric with drying of residue			4500-SO4 2− D	4500-SO4 2− D	4500-SO4 2− D-97.
	Turbidimetric method		D 516-07, 11, 16	4500-SO4 2− E	4500-SO4 2− E	4500-SO4 2− E-97.
	Automated methylthymol blue method			4500-SO4 2− F	4500-SO4 2− F	4500-SO4 2− F-97.
Total Dissolved Solids	Total Dissolved Solids Dried at 180 deg C			2540 C	2540 C.
Zinc	Axially viewed inductively coupled plasma-atomic emission spectrometry (AVICP-AES)	200.5, Revision 4.2.²
	Atomic Absorption; Direct Aspiration			3111 B	3111 B.
	Inductively Coupled Plasma			3120 B	3120 B.

¹Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street, NW., Washington, DC 20001-3710.

²EPA Method 200.5, Revision 4.2. “Determination of Trace Elements in Drinking Water by Axially Viewed Inductively Coupled Plasma-Atomic Emission Spectrometry.” 2003. EPA/600/R-06/115. (Available at http://www.epa.gov/water-research/epa-drinking-water-research-methods.)

³Standard Methods Online are available at http://www.standardmethods.org. The year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only online versions that may be used.

⁴Available from ASTM International, 100 Barr Harbor Drive, West Conshohocken, PA 19428-2959 or http://astm.org. The methods listed are the only alternative versions that may be used.

⁵Method D99-003, Revision 3.0. “Free Chlorine Species (HOCl− and OCl−) by Test Strip,” November 21, 2003. Available from Industrial Test Systems, Inc., 1875 Langston St., Rock Hill, SC 29730.

⁶Standard Methods for the Examination of Water and Wastewater, 20th edition (1998). Available from American Public Health Association, 800 I Street, NW., Washington, DC 20001-3710.

⁷Method ME355.01, Revision 1.0. “Determination of Cyanide in Drinking Water by GC/MS Headspace,” May 26, 2009. Available at http://www.nemi.gov or from James Eaton, H & E Testing Laboratory, 221 State Street, Augusta, ME 04333. (207) 287-2727.

⁸Systea Easy (1-Reagent). “Systea Easy (1-Reagent) Nitrate Method,” February 4, 2009. Available at http://www.nemi.gov or from Systea Scientific, LLC., 900 Jorie Blvd., Suite 35, Oak Brook, IL 60523.

⁹EPA Method 524.3, Version 1.0. “Measurement of Purgeable Organic Compounds in Water by Capillary Column Gas Chromatography/Mass Spectrometry,” June 2009. EPA 815-B-09-009. Available at https://www.nemi.gov.

¹⁰Mitchell Method M5271, Revision 1.1. “Determination of Turbidity by Laser Nephelometry,” March 5, 2009. Available at http://www.nemi.gov or from Leck Mitchell, PhD, PE, 656 Independence Valley Dr., Grand Junction, CO 81507.

¹¹Mitchell Method M5331, Revision 1.1. “Determination of Turbidity by LED Nephelometry,” March 5, 2009. Available at http://www.nemi.gov or from Leck Mitchell, PhD, PE, 656 Independence Valley Dr., Grand Junction, CO 81507.

¹²Orion Method AQ4500, Revision 1.0. “Determination of Turbidity by LED Nephelometry,” May 8, 2009. Available at http://www.nemi.gov or from Thermo Scientific, 166 Cummings Center, Beverly, MA 01915, http://www.thermo.com.

¹³Modified Colitag^TM Method, “Modified Colitag^TM Test Method for the Simultaneous Detection of E. coli and other Total Coliforms in Water (ATP D05-0035),” August 28, 2009. Available at http://www.nemi.gov or from CPI, International, 580 Skylane Boulevard, Santa Rosa, CA 95403.

¹⁴EPA Method 557. “Determination of Haloacetic Acids, Bromate, and Dalapon in Drinking Water by Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC-ESI-MS/MS),” September 2009. EPA 815-B-09-012. Available at https://www.nemi.gov.

¹⁵AMI Turbiwell, “Continuous Measurement of Turbidity Using a SWAN AMI Turbiwell Turbidimeter,” August 2009. Available at http://www.nemi.gov or from Markus Bernasconi, SWAN Analytische Instrumente AG, Studbachstrasse 13, CH-8340 Hinwil, Switzerland.

¹⁶EPA Method 334.0. “Determination of Residual Chlorine in Drinking Water Using an On-line Chlorine Analyzer,” September 2009. EPA 815-B-09-013. Available at https://www.nemi.gov.

¹⁷ChloroSense. “Measurement of Free and Total Chlorine in Drinking Water by Palintest ChloroSense,” August 2009. Available at https://www.nemi.gov or from Palintest Ltd, 1455 Jamike Avenue (Suite 100), Erlanger, KY 41018.

¹⁸EPA Method 302.0. “Determination of Bromate in Drinking Water using Two-Dimensional Ion Chromatography with Suppressed Conductivity Detection,” September 2009. EPA 815-B-09-014. Available at https://www.nemi.gov.

¹⁹EPA 415.3, Revision 1.2. “Determination of Total Organic Carbon and Specific UV Absorbance at 254 nm in Source Water and Drinking Water,” September 2009. EPA/600/R-09/122. Available at http://www.epa.gov/water-research/epa-drinking-water-research-methods.

²⁰Readycult® Method, “Readycult® Coliforms 100 Presence/Absence Test for Detection and Identification of Coliform Bacteria and Escherichia coli in Finished Waters,” January, 2007. Version 1.1. Available from EMD Millipore (division of Merck KGaA, Darmstadt, Germany), 290 Concord Road, Billerica, MA 01821.

²¹Chromocult® Method, “Chromocult® Coliform Agar Presence/Absence Membrane Filter Test Method for Detection and Identification of Coliform Bacteria and Escherichia coli in Finished Waters,” November, 2000. Version 1.0. EMD Millipore (division of Merck KGaA, Darmstadt, Germany), 290 Concord Road, Billerica, MA 01821.

²²Hach Company. “Hach Company SPADNS 2 (Arsenite-Free) Fluoride Method 10225—Spectrophotometric Measurement of Fluoride in Water and Wastewater,” January 2011. 5600 Lindbergh Drive, P.O. Box 389, Loveland, Colorado 80539.

²³Hach Company. “Hach Company TNTplus^TM 835/836 Nitrate Method 10206—Spectrophotometric Measurement of Nitrate in Water and Wastewater,” January 2011. 5600 Lindbergh Drive, P.O. Box 389, Loveland, Colorado 80539.

²⁴EPA Method 525.3. “Determination of Semivolatile Organic Chemicals in Drinking Water by Solid Phase Extraction and Capillary Column Gas Chromatography/Mass Spectrometry (GC/MS),” February 2012. EPA/600/R-12/010. Available at http://www.epa.gov/water-research/epa-drinking-water-research-methods.

²⁵EPA Method 536. “Determination of Triazine Pesticides and their Degradates in Drinking Water by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC/ESI-MS/MS),” October 2007. EPA 815-B-07-002. Available at the National Service Center for Environmental Publications (EPA Method 536).

²⁶EPA Method 523. “Determination of Triazine Pesticides and their Degradates in Drinking Water by Gas Chromatography/Mass Spectrometry (GC/MS),” February 2011. EPA 815-R-11-002. Available at the National Service Center for Environmental Publications (EPA Method 523).

²⁷EPA Method 1623.1. “Cryptosporidium and Giardia in Water by Filtration/IMS/FA,” 2012. EPA-816-R-12-001. Available at the National Service Center for Environmental Publications (www.epa.gov/nscep). Search “816R12001”.

²⁸Standard Methods for the Examination of Water and Wastewater, 22nd edition (2012). Available from American Public Health Association, 800 I Street NW., Washington, DC 20001-3710.

²⁹EPA Method 524.4, Version 1.0. “Measurement of Purgeable Organic Compounds in Water by Gas Chromatography/Mass Spectrometry using Nitrogen Purge Gas,” May 2013. EPA 815-R-13-002. Available at the National Service Center for Environmental Publications (EPA Method 524.4).

³⁰Charm Sciences Inc. “Fast Phage Test Procedure. Presence/Absence for Coliphage in Ground Water with Same Day Positive Prediction”. Version 009. November 2012. 659 Andover Street, Lawrence, MA 01843. Available at www.charmsciences.com.

³¹Hach Company. “Hach Method 10260—Determination of Chlorinated Oxidants (Free and Total) in Water Using Disposable Planar Reagent-filled Cuvettes and Mesofluidic Channel Colorimetry,” April 2013. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

³²ChlordioX Plus. “Chlorine Dioxide and Chlorite in Drinking Water by Amperometry using Disposable Sensors,” November 2013. Available from Palintest Ltd, Jamike Avenue (Suite 100), Erlanger, KY 41018.

³³Tecta EC/TC. “Techta^TMEC/TC Medium and Techta^TMInstrument: A Presence/Absence Method for the Simultaneous Detection of Total Coliforms and Escherichia coli (E. coli) in Drinking Water,” version 1.0, May 2014. Available from Pathogen Detection Systems, Inc., 382 King Street East, Kingston, Ontario, Canada, K7K 2Y2.

³⁴Hach Company. “Hach Method 10241—Spectrophotometric Measurement of Free Chlorine (Cl2) in Drinking Water,” November 2015. Revision 1.2. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

³⁵Hach Company. “Hach Method 8026—Spectrophotometric Measurement of Copper in Finished Drinking Water,” December 2015. Revision 1.2. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

³⁶Hach Company. “Hach Method 10272—Spectrophotometric Measurement of Copper in Finished Drinking Water,” December 2015. Revision 1.2. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

³⁷Hach Company. “Hach Method 10261—Total Organic Carbon in Finished Drinking Water by Catalyzed Ozone Hydroxyl Radical Oxidation Infrared Analysis,” December 2015. Revision 1.2. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

³⁸Hach Company. “Hach Method 10267—Spectrophotometric Measurement of Total Organic Carbon (TOC) in Finished Drinking Water,” December 2015. Revision 1.2. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

³⁹Hach Company. “Hach Method 10258—Determination of Turbidity by 360° Nephelometry,” January 2016. Revision 1.0. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

⁴⁰Nitrate Elimination Company, Inc. (NECi). “Method for Nitrate Reductase Nitrate-Nitrogen Analysis of Drinking Water,” February 2016. Superior Enzymes, Inc., 334 Hecla Street, Lake Linden, Michigan 49945.

⁴¹Thermo Fisher. “Thermo Fisher Scientific Drinking Water Orthophosphate Method for Thermo Scientific Gallery Discrete Analyzer,” February 2016. Revision 5. Thermo Fisher Scientific, Ratastie 2, 01620 Vantaa, Finland.

⁴²Mitchell Method M5331, Revision 1.2. “Determination of Turbidity by LED or Laser Nephelometry,” February 2016. Available from Leck Mitchell, Ph.D., PE, 656 Independence Valley Dr., Grand Junction, CO 81507.

⁴³Tecta EC/TC. “TectaTM EC/TC Medium and the TectaTM Instrument: A Presence/Absence Method for the Simultaneous Detection of Total Coliforms and Escherichia coli (E. coli) in Drinking Water,” version 2.0, February 2017. Available from Pathogen Detection Systems, Inc., 382 King Street East, Kingston, Ontario, Canada, K7K 2Y2.

⁴⁴Lovibond PTV 1000. “Continuous Measurement of Drinking Water Turbidity Using a Lovibond PTV 1000 White Light LED Turbidimeter,” December 2016. Revision 1.0. Available from Tintometer, Inc., 6456 Parkland Drive, Sarasota, FL 34243.

⁴⁵Lovibond PTV 2000. “Continuous Measurement of Drinking Water Turbidity Using a Lovibond PTV 2000 660-nm LED Turbidimeter,” December 2016. Revision 1.0. Available from Tintometer, Inc., 6456 Parkland Drive, Sarasota, FL 34243.

⁴⁶Lovibond PTV 6000. “Continuous Measurement of Drinking Water Turbidity Using a Lovibond PTV 6000 Laser Turbidimeter,” December 2016. Revision 1.0. Available from Tintometer, Inc., 6456 Parkland Drive, Sarasota, FL 34243.

⁴⁷Thermo Fisher. “Thermo Fisher Method 557.1: Determination of Haloacetic Acids in Drinking Water using Two-Dimensional Ion Chromatography with Suppressed Conductivity Detection,” January 2017. Version 1.0. Available from Thermo Fisher Scientific, 490 Lakeside Dr., Sunnyvale, CA 94085 ([email protected]).

⁴⁸EPA Method 150.3. “Determination of pH in Drinking Water,” February 2017. EPA 815-B-17-001. Available at the National Service Center for Environmental Publications (EPA Method 150.3).

⁴⁹Standard Methods for the Examination of Water and Wastewater, 23rd edition (2017). Available from American Public Health Association, 800 I Street NW, Washington, DC 20001-3710.

⁵⁰EPA Method 900.0, Rev. 1.0. “Determination of Gross Alpha and Gross Beta in Drinking Water,” February 2018. EPA 815-B-18-002. Available at the National Service Center for Environmental Publications (EPA Method 900.0 Rev 1.0).

⁵¹Hach Company. “Hach Method 10258—Determination of Turbidity by 360° Nephelometry,” March 2018. Revision 2.0. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

⁵²Hach Company. “Hach Method 8195—Determination of Turbidity by Nephelometry,” March 2018. Revision 3.0. 5600 Lindbergh Drive, P.O. Box 389, Loveland, CO 80539.

[74 FR 38353, Aug. 3, 2009, as amended at 74 FR 57914, Nov. 10, 2009; 74 FR 63069, Dec. 2, 2009; 75 FR 32299, June 8, 2010; 76 FR 37018, June 24, 2011; 77 FR 38527, June 28, 2012; 78 FR 32565, May 31, 2013; 78 FR 37463, June 21, 2013; 79 FR 35086, June 19, 2014; 79 FR 36428, June 27, 2014; 81 FR 46844, July 19, 2016; 82 FR 34867, July 27, 2017; 83 FR 51644, Oct. 12, 2018; 83 FR 54676, Oct. 31, 2018]

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