9 CFR §113.32
Verified against eCFR.gov as of June 20, 2026View official text on eCFR.gov ↗
The test for detection of Brucella contamination provided in this section shall be conducted when such a test is prescribed in an applicable Standard Requirement or in a filed Outline of Production for the product.
- (a)One ml of the minced tissue used as the source of cells or 1 ml of the extract of the tissue prior to the addition of antibiotics, diluent and stabilizer, shall be inoculated onto each of three tryptose agar plates and incubated in a 10 percent CO2 atmosphere at a temperature of 35-37 °C for at least 7 days.
- (b)If colonies are identified as Brucella, the biological product is unsatisfactory.
- (c)If colonies suspicious of Brucella are observed but cannot be identified as a Brucella species, either